Chikungunya Vaccine Candidates: Current Landscape and Future …
WEBOct 20, 2022 · An mRNA vaccine encoding chikungunya virus E2-E1 protein elicits robust neutralizing antibody responses and CTL immune responses. Virol Sin. 2022; 37 (2):266–276. doi: 10.1016/j.virs.2022.01.032 [PMC free article] [Google Scholar]
Cellular Attachment and Entry Factors for Chikungunya Virus
WEBNov 19, 2019 · Heterodimers of the E1 and E2 proteins assemble into spikes on the virion surface and facilitate the infection of target cells [1]. The E1 protein contains a hydrophobic fusion peptide and is necessary for viral and cellular membrane fusion.
Emerging Chikungunya Virus Variants at the E1-E1 ... - PubMed
WEBFeb 23, 2022 · Using an in vivo transmission system that allows intrahost evolution, we identified an emerging CHIKV variant carrying a mutation in the E1 glycoprotein (V156A) in the serum of mice and saliva of mosquitoes.
Emerging Chikungunya Virus Variants at the E1-E1 …
WEBUsing an in vivo transmission system that allows intrahost evolution, we identified an emerging CHIKV variant carrying a mutation in the E1 glycoprotein (V156A) in the serum of mice and saliva of mosquitoes.
Expression, Purification, and Refolding of Chikungunya Virus Full ...
WEBFeb 1, 2022 · The envelope E2 protein is one of the promising subunit vaccine candidates against CHIKV. In this study, we describe successful cloning, expression, and purification of CHIKV E2 full-length (E2-FL) and truncated (E2-ΔC and E2-ΔNC) proteins in the Escherichia coli expression system.
Chikungunya fever - Nature Reviews Disease Primers
WEBApr 6, 2023 · E2 and E1 are type I transmembrane proteins and form heterodimers, three of which form a trimeric spike; 80 of these trimeric spikes are presented on the viral particle.
Chikungunya Virus Diagnosis: A Review of Current Antigen
WEBJul 17, 2023 · The E1/E2 CHIKV ELISA was validated using 100 PCR-confirmed Chikungunya samples and 60 negative samples from Honduras. The results revealed that the performance of the ELISA ranged from 41.33% to 95.45% in sensitivity and 84.76% to 98.02% in specificity over the Ct range of 20 to 35.
An mRNA vaccine encoding Chikungunya virus E2-E1 protein
WEBHere, we developed an mRNA-lipid nanoparticle (mRNA-LNP) vaccine expressing CHIKV E2-E1 antigen, and compared its immunogenicity with soluble recombinant protein sE2-E1 antigen expressed in S2 cells. For comparison, we first showed that recombinant protein antigens mixed with aluminum adjuvant elicit strong antigen-specific humoral immune ...
Chikungunya virus cell-to-cell transmission is mediated by ...
WEBAug 17, 2023 · MXRA8 is an attachment and entry receptor for CHIKV that binds to E2/E1 heterodimers on the virus particle and promotes infection in cell culture and in mice 26,27.
An mRNA vaccine encoding Chikungunya virus E2-E1
WEBApr 1, 2022 · To test the mRNA platform in the development of CHIKV vaccine, we constructed an mRNA vaccine expressing the E2-E1 heterodimer protein (mRNA-E2-E1) (Fig. 3 A), and first examined the protein expression by …